Vitamin D | Pathology Tests Explained

Vitamin D is a nutrient that is important for healthy bones, muscles and teeth. It helps your body absorb calcium. Having enough vitamin D may reduce your chance of developing many common conditions.
Vitamin D can be found in two forms:
Vitamin D2 and vitamin D3 need to be turned into the hormone 25-hydroxy-vitamin D to function effectively in your body. This is done by your liver and kidneys.
The vitamin D blood test measures how much 25-hydroxy-vitamin D is available in your body.
Other forms of vitamin D can also be measured if needed.
If you do not have enough vitamin D, your doctor may recommend you take a supplement.
A vitamin D test might be part of a check-up for people at risk of having low vitamin D levels (deficiency), such as people who:
Your doctor might also recommend this test if you have:
If you have low vitamin D levels and a young baby, your doctor may refer your child for a blood test to check their levels.
You usually need to go to a pathology collection centre to get a vitamin D blood test. Your doctor will recommend one near you. You need a referral to get this blood test.
Sometimes, your doctor will collect blood for testing in their clinic. Blood tests are also routinely performed in hospitals.
No special preparation is required for a vitamin D blood test.
If you are having other blood tests done at the same time, there may be special instructions you need to follow. It's best to check with your doctor or the pathology collection centre. Ask them if there is anything you need to do before having your blood test.
A health professional will take a sample of blood from a vein in your arm. The blood will then be sent to a laboratory for testing.
The 'normal range' for vitamin D levels in the general population is not yet fully defined, and different laboratories may show varying normal ranges.
A low level of 25-hydroxy-vitamin D may mean:
A high level of vitamin D could mean:
Vitamin D levels can be higher in summer than in winter.
Talk to your doctor about what your results mean for you.
Go to the Pathology Tests Explained website for more information about Vitamin D testing (Opens in new tab).
Read the Royal Australian College of General Practitioners' (RACGP) patient fact sheet on Vitamin D testing (Opens in new tab).
Visit Healthy Bones Australia (Opens in new tab) to learn about bone health and osteoporosis.
See healthdirect's 'Guide to blood testing' to learn more about blood tests in general including information such as:
You can also call the healthdirect helpline on 1800 022 222 (known as NURSE-ON-CALL in Victoria). A registered nurse is available 24 hours a day, 7 days a week.'
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Last reviewed: March 2025



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