Thyroid Function Tests | Pathology Tests Explained

Thyroid function tests are used to check for thyroid problems. These tests measure your blood levels of:
The pituitary gland in your brain makes TSH and sends it to your thyroid gland. TSH controls the production of thyroid hormones T3 and T4.
TSH is usually tested first. If your results are outside of the normal range (too high or too low), your doctor may request tests for T3 and T4. A test that checks your levels of TSH, T3 and T4 is known as a thyroid function test (TFT).
Additional tests your doctor may consider if they are concerned about a thyroid condition include:
Your thyroid gland has an important role in controlling your metabolism. Hormones made by your thyroid gland influence your:
Too low or too high levels of these hormones can make you unwell.
Sometimes your thyroid hormones can be outside of the normal range if you have a problem with another endocrine (hormone-producing) organ. Thyroid function tests can help find out if your problem is with the thyroid gland, rather than the pituitary or hypothalamus.
Find out more about thyroid conditions.
Your doctor might recommend thyroid function tests if you:
All newborn babies in Australia are screened for some types of thyroid problems using the heel prick (Opens in new tab) test. One in every 4,000 babies is born without a working thyroid gland.
You usually need to go to a pathology collection centre with a referral from your doctor. Your doctor will recommend one near you.
Sometimes, your doctor will collect blood for testing in their clinic. Blood tests are also routinely done in hospitals.
You don't need to prepare for a TSH blood test.
Tell your doctor if you are taking any medicines or supplements, especially medicine for thyroid problems.
If you are having other blood tests done at the same time, you may need to follow other instructions. Check with your doctor or the pathology collection centre if there is anything you need to do before your blood test.
A health professional will take a sample of blood from a vein in your arm. The blood will then be sent to a laboratory for testing.
A high TSH result might mean that you:
A low TSH result might mean that you:
If your TSH test result isn't normal, your doctor may ask the lab to test your thyroxine (T4 (Opens in new tab)) and/or triiodothyronine (T3 (Opens in new tab)) levels.
Discuss your test results with your doctor to find out what they mean for you.
See Pathology Tests Explained (Opens in new tab) for more information about TSH, T3 and T4 blood tests and thyroid problems.
Read more about the thyroid and thyroid problems at the Australian Thyroid Foundation (Opens in new tab).
Visit healthdirect's 'Guide to blood testing' to learn more about blood tests in general such as:
You can call the healthdirect helpline on 1800 022 222 (known as NURSE-ON-CALL in Victoria). A registered nurse is available 24 hours a day, 7 days a week.
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Last reviewed: March 2025










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